次世代定序服務
Next Generation Sequencing Services
Phalanx Biotech Group使用Illumina / BGI平台提供NGS服務，協助您取得一系列的生物實驗數據，包含基因組學(Genomics)、轉錄組學(Transcriptomics)、表觀遺傳學(Epigenomics)和宏觀基因組學(Metagenomics)等。我們可以依照您的需求，提供您實驗設計、檢體準備、定序、分析…等的各種協助。
NGS服務
RNA-Seq
RNA-Seq Service
RNA sequencing is a type of next-generation sequencing used to target regions of the genome that are transcribed into
messenger RNA (mRNA) and may subsequently translate into protein. RNA-seq is used to identify and quantify
transcriptional regulation of the genome.
Protocols specific to the library preparation stage are used to isolate mRNA and rid samples of rRNA, which comprises
approximately 80% of the RNA pool.
Typical sequencing configurations:
| Configuration | mRNA-seq |
|---|---|
| Library Preparation Protocol | Strand-specific, polyA Selection, paired-end |
| Recommended Throughput | 20-40 million reads per sample |
| Platform | Illumina HiSeq |
*Sequencing configuration is project specific.
Sample submission requirements for RNA-Sequencing:
| SAMPLE TYPE | AMOUNT | RIN | CONCENTRATION |
|---|---|---|---|
| Total RNA in Rnase-free water, on dry ice | 2ug (min > 500ng) | 6.0 | 50 ng/uL |
RNA-Seq Applications:
RNA-seq may be used for gene expression profiling, identification of novel transcripts, identification of alternative splice events and transcriptome assembly.
Gene expression profiling by way of RNA-seq is utilized in drug development experiments, cancer research, gut microbiome studies and many more. Comparative analysis of transcript abundance between experimental groups provides unique insight into the interplay between gene and environment.
Small RNA-Seq
Small RNA-Seq Service
Small RNA sequencing is used to target small RNA molecules including: miRNA, ncRNA, snRNA, etc.
Common sequencing configurations:
| Configuration | small RNA-seq |
|---|---|
| Library Preparation Protocol | Customizable size selection using small RNA library prep kits |
| Recommended Throughput | 7-10 million reads per sample |
| Platform | Illumina NextSeq |
Sample submission requirements for Small RNA-Sequencing:
| Sample Type | Minimum Amount | RIN | Minimum Concentration |
|---|---|---|---|
| Total RNA | 200 ng | >7.0 | 20 g/uL |
ChIP-Seq
ChIP-Seq Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 5 ng | 1 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- Single end 1x50 bp
- 20M, 40M, 75M, or 100M reads
16s Metagenomics
Smple submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- Paired end 2x300bp
- 100K coverage
Whole Exome Sequencing (WES)
Sample submission requirements:
| Sample Type | Minimum Amount | Minimum Concentration |
|---|---|---|
| DNA | 12.5 ng | 1.5 ng/uL |
RNA QC Requirements:
- A260/280 > 2
- 260/230 = 2.0~2.2
Recommended Sequencing Specifications:
- aired end 2x300 bp
- 100K coverage
特色
- 完整的工作流程：我們可以依照您的需求，執行樣品核酸提取、建庫(library construction)、定序、基礎數據分析和進階數據分析。
- 低產量樣品：如果您的樣品類型為有限的RNA / DNA，請聯繫我們以了解我們能提供的特殊專屬流程。
- 種類繁多：我們處理過各樣基因組（人類，小鼠，大鼠，倉鼠，細菌等）物種的樣本。
資源
對產品有任何問題皆歡迎提出，我們會盡快與您聯繫！
或洽詢服務專線+886-3-578-1168；0800-777988(按0)，由專人為您提供諮詢！